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"Education is the most powerful weapon which you can use to change the world”
– Nelson Mandela

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Laureate Education is a global network of higher education institutions, founded in 1999 with the mission of providing easier access to quality academic programs in fields such as business, medical, l...

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In adoption process children should be given a proper secured family. The fact that the family which adopts the children are well settled them, the parents are ready to not only take the responsibilit...

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Lab 6 Part 1

Lab 6 Part 1

Q Unknown Lab Report- Lab 13 • Points 20 • Submitting a text entry box or a file upload For your Unknown Lab, include the following information: ±Include drawings of your gram-negative unknown bacteria on all three plates: TSA, MacConkey, and EMB. Make sure to describe colony morphology, including color and colony size. ±Draw cell morphology and include cell size ±Write an overview of each biochemical test and what your test results were. ±Prepare a table that includes your observations and interpretations for each test. ±Draw pictures for each test. ±Write a conclusion section that explains your choice and discusses any inconsistent results. ±Research your unknown bacteria and include a paragraph about it. ±Include the summary sheet.

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To identify the unknown organism, we first need to obtain a pure culture of the organism. Once the pure culture is obtained, it can be subjected to Gram staining. • Gram Staining is done to differentiate bacteria into two groups based on the difference in their cell wall composition. Two dyes, namely, crystal violet (primary stain) and safranine (counterstain), are used along with iodine (as a mordant) and 70% ethanol (as decolourizing agent). The cell wall of Gram-negative bacteria contains large amounts of lipids. When the decolourizing agent is used, the violet color is removed from the cell wall of Gram-negative cells. When viewed under the microscope, Gram-positive organisms appear purple while Gram-negative ones appear pink in color.